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Thus, we reached the preliminary conclusion that out of the entire AtCaM genes investigated, AtCaM1 and AtCaM4, which encode the same protein , doubtless function within the response of Arabidopsis to salt. CaM is the most important multifunctional Ca2+ sensor in eukaryotes. The structure and function of plant CaMs are similar to these of animal and yeast CaMs; however, plant genomes include a number of CaM genes that encode equivalent CaM isoforms (about 6–12) . The existence of comparable amino acid sequences among isoforms is a distinguishing characteristic of upper plants . Given this, figuring out which CaM isoforms are conscious of salt was a main focus of the current research. CaM consists of soluble single-chain proteins, each consisting of two globular domains related by an α-helical linker.
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- Arabidopsis transformation with Agrobacterium tumefaciens was performed by the floral dip methodology .
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CaM is also regarded as concerned in salt stress signaling. The expression of CaM in sweet potato (Ipomoea batatas L.) is induced by NaCl . A particular CaM isoform mediates salt-induced Ca2+ signaling through the activation of a MYB transcriptional activator, leading to salt tolerance in crops . Overexpression of GmCaM4 in soybean (Glycine max L.) enhances plant resistance to pathogens and tolerance to salt stress . However, direct proof of the participation of CaM in salt tolerance and its corresponding signaling pathway in vivo is missing. Additional studies are wanted to acquire new insight into the salt signaling network.
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